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KMID : 0545120120220101375
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1375 ~ p.1380
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Functional Mechanism of Plant Growth Retardation by Bacillus subtilis IJ-31 and Its Allelochemicals
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Kim Won-Chan
Rhee In-Koo
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Abstract
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We previously isolated a rhizobacterium (Bacillus subtilis IJ-31) and demonstrated that its associated allelochemicals could indicate plant growth retardation. However, little is known about how the growth of plants is regulated by B. subtilis IJ-31 and its allelochemicals. In this study, we investigated whether plant growth retardation in this relationship occurred through the inhibition of gibberellin (GA) biosynthesis. GA 3β-hydroxylase activity was found to be inhibited by B. subtilis IJ-31 and hydrocinnamic acid (HCA), which is one of the allelochemicals produced by B. subtilis IJ-31. Additionally, thin layer chromatography (TLC) demonstrated that B. subtilis IJ-31 culture broth and HCA both inhibit GA 3β-hydroxylase (MBP-GA4) activity. The retardation of plants by HCA was then confirmed in vivo and in vitro using a Ryegrass and Arabidopsis growth retardation assay. Furthermore, treatment with either B. subtilis IJ-31 culture extract or its allelochemicals resulted in significant down-regulation of XTR9 gene expression in Arabidopsis. Overall, we identified the functional mechanism of plant growth retardation by B. subtilis IJ-31 and its allelochemicals.
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KEYWORD
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Bacillus subtilis, gibberellin 3β-hydroxylase, allelochemicals, hydrocinnamic acid, Xyloglucan endotransglycosylases
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